Regulatory

Part:BBa_K5082008:Design

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-China   (2024-09-07)


pCMV-EGFP plasmid vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1630
    Illegal XbaI site found at 1667
    Illegal SpeI site found at 216
    Illegal PstI site found at 1635
    Illegal PstI site found at 2989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1630
    Illegal NheI site found at 862
    Illegal SpeI site found at 216
    Illegal PstI site found at 1635
    Illegal PstI site found at 2989
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1630
    Illegal BglII site found at 1610
    Illegal BglII site found at 6116
    Illegal BamHI site found at 1661
    Illegal XhoI site found at 1614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1630
    Illegal XbaI site found at 1667
    Illegal SpeI site found at 216
    Illegal PstI site found at 1635
    Illegal PstI site found at 2989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1630
    Illegal XbaI site found at 1667
    Illegal SpeI site found at 216
    Illegal PstI site found at 1635
    Illegal PstI site found at 2989
    Illegal NgoMIV site found at 2099
    Illegal NgoMIV site found at 3440
    Illegal NgoMIV site found at 3723
    Illegal AgeI site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 849
    Illegal BsaI.rc site found at 5248
    Illegal SapI site found at 4168
    Illegal SapI.rc site found at 3289
    Illegal SapI.rc site found at 3499


Design Notes

1


Source

1

References